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| 产品名称： | MOLM-16 |
|---|---|
| 商品货号： | TS259849 |
| <strong>Cell line </strong>： | MOLM-16 |
| DSMZ no. ： | TS259849 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1999 from the peripheral blood of a 77-year-old Japanese woman at relapse of acute myeloid leukemia (AML M0) after failed chemotherapy |
| Reference(s) ： | 14747 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large round cells growing singly or in small clumps in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well-plate; later maintain at ca 0.2-0.8 x x106 cells/ml; split saturated culture 1:2 to 1:4 every 2-5 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-80 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD19 -, CD33 +, CD71 +, CD235a +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with hypodiploid sideline - 83-88<4n>XXX, -X, -4, -4, -5, +6, +7, -9, -18, +2mar, add(5)(q11)x2, add(6)(q11-13), der6)t(6;9(q16;p13), t(6;8)(q15;q24.3), der(8)t(6;8)(q15;q24.3), der(9;18)(p10;q10), der(16)del(16)(p13)add(16)(q11), der(16)(del16)(p13)dup(16) (q?11q?24)ins(16;?)(q11;??), der(18)t(9;18)(q13;q21), der(19)add(19)(p13)add(19)(q13)x2 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |