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| 产品名称： | ROS-50 |
|---|---|
| 商品货号： | TS259850 |
| <strong>Cell line </strong>： | ROS-50 |
| DSMZ no. ： | TS259850 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | B cell leukemia |
| Origin ： | established from the peripheral blood of a 69-year-old man with B cell acute lymphoblastic leukemia (B-ALL L3) at relapse; reported to carry the BCL2 rearrangement in the minor cluster region (mcr) in contrast to the major breakpoint region (MBR) of most cell lines |
| Reference(s) ： | 14498, 15041 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly or in clusters in suspension; image; image; image; image |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | seed out at ca. 1-1.5 x 106 cells/ml; cells may be difficult to culture; after thawing cells may need 5-7 days to recover completely and need dense contact for optimal proliferation; in exponential growth split culture 1:2 to 1:3 every 2-3 days; maintain at 0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 1.5-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 45-48<2n>XY, +7, +8, der(7;17)(p10;q10), t(8;14;18)(q24;q32;q21), der(8)t(8;14;18) - carries rearrangements of BCL2, IGH and MYC - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |