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| 产品名称： | NCI-H82 |
|---|---|
| 商品货号： | TS259851 |
| <strong>Cell line </strong>： | NCI-H82 |
| DSMZ no. ： | TS259851 |
| Species ： | confirmed as human by PCR speciation |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established from the pleural effusion of a 41-year-old white man with small cell cancer of the lung in 1978 |
| Reference(s) ： | 14849 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells growing in clumps in suspension |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca 0.1-0.8 x 106 cells/ml; cell numbers can only be estimated due to the formation of large aggregates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV - |