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| 产品名称： | JEKO-1 |
|---|---|
| 商品货号： | TS259857 |
| <strong>Cell line </strong>： | JEKO-1 |
| DSMZ no. ： | TS259857 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood of a 78-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), specifically mantle cell lymphoma, large variant in leukemic conversion. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14490, 14639, 15850, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells growing in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.5 x106 cells/ml; split saturated culture 1:4 twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 1.7 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylamda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypertriploid karyotype - 70-78<3n>XXXX, +X, +1, +2, +2, +4, +6, +7, -8, +10, +11, -12, -12, +13, +14, +15, -17, +18, -19, -19, -22, +2-4mar, del(X)(p21)/add(X)(p11.2)x2, der(X)t(X;11)(q26-28;q13)t(11;?10)(q24;?p12), der(1)t(1;2)(p21;q22)x2, add(2)(q2?), del(2)(q11), add(3)(q27), der(5)t(5;12)(p15;p11-12)x2, add(6)(p21)x2, der(6;13)(p10;q10)x1-2, der(7)t(4;7)(q2?6;q32.1)x2, add(9)(p2?), der(9)t(9;14)(q34-35;q32-33)x2, der(10)t(10;11)(p12;?)x2, der(11)t(11;14)(q13;q32)x1-2, add(13)(p11), der(13)t(13;?)(p11;?)t(?;13)(?;q11), add(14)(p11), der(14)t(14;17)(p12;q11)t(14;6)(q24;?p11), der(14)t(11;14)(?;p11)t(11;?8)(?;?), del(16)(p11), add(17)(p11), ins(19;12)(q13.1;q13.2q2?4)x2 - carries cryptic rearrangements of CCND1 and IGH (IGH) - related to published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |