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| 产品名称： | RC-K8 |
|---|---|
| 商品货号： | TS259859 |
| <strong>Cell line </strong>： | RC-K8 |
| DSMZ no. ： | TS259859 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peritoneal effusion of a patient with lymphoma, described at the time as true histiocytic lymphoma, in 1984 (terminal, refractory stage); assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14693, 14694, 14629, 17355 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing mainly in suspension or in small clusters; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | split saturated culture 1:3 to 1:5 every 3-4 days; seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at 0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 4% polyploidy - 47(42-49)<2n>XY, -13, -15, +2 mar, der(Y)t(Y;5)(qter;?p15), der(?1)t(1;13)(p32;q11)del(1)(q11), del(2)(p11p13), dup(2)(p13p16), add(3)(q2?), der(5)?inv(5)(q15q32)add(5)(q32), inv(5)(p?13q?32), add(7)(q31), der(8)(t(8;8)(p12;q11), del(9)(p24)x2, der(10)t(9;10)(?p24;p11), t(11;14)(q23;q32), der(14)t(X;14)(p11;p11), der(15)(t(X;15)(p11.3;p11), add(17)(p11), der(20)t(13;20)(q11;q13) - carries breakpoints at REL and IGH-DDX6 - matches published karyotype |
| Molec. Genetics ： | BCL6 translocation was verified by genomic PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |