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| 产品名称： | HCC-78 |
|---|---|
| 商品货号： | TS259861 |
| <strong>Cell line </strong>： | HCC-78 |
| DSMZ no. ： | TS259861 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | non-small cell lung carcinoma |
| Origin ： | established from the pleural effusion of a 65-year-old man with adenocarcinoma of the lung, typed as non-small cell lung carcinoma; cells are described in the literature to carry a ROS1 rearrangement; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-78BL) |
| Reference(s) ： | 15046, 14567, 19109 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large epitheloid cells growing in monolayers and aggregates; after trypsination cells are not always dispersed as single cells; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out 1-2 x 106 cells in 175 cm2; split confluent culture 1:2 twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded complex hypotetraploid karyotype with 6% polyploidy - 80-91<4n>XYY/XXYY, add(X)(q25)x2, add(1)(?p11), add(4)(p15), add(4)(q31), add(16)(q23), der(16)t(1;16)(p12;q23)x2, dup(16)(q11q13)add(16)(q13), add(19)(q13)x2, inc. |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |