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| 产品名称： | EJM |
|---|---|
| 商品货号： | TS259862 |
| <strong>Cell line </strong>： | EJM |
| DSMZ no. ： | TS259862 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | multiple myeloma |
| Origin ： | established from the peritoneal fluid of a 58-year-old woman with IgG lambda myeloma in 1988 at her terminal refractory stage |
| Reference(s) ： | 14596 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph, single or clustered cells growing either in suspension or adherent; image; image; image |
| Medium ： | 80-90% Iscoves MDM + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 2-3 x 10 6 cells/80 cm2; split saturated culture 1:2 every 2-3 days (using trypsin/EDTA for the adherent cells); viability may drop below 60% upon thawing, but cells recover within one week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, cyIgG +, cyIgM -, cykappa -, cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypotriploid karyotype - 62(50-62)<3n>X, -X, -X, +3, -4, +5, -6, +9, -10, -12, -13, -14, -15, -16, +19, -21, +22, der(?)t(X;?)(q11;?)x2, add(3)(p1?1), add(3)(p22-24), add(3)(q11), der(3)t(3;15)(p11;q11)del(3)(p25), add(4)(p11)del(4)(q2?4q3?3), add(5)(q11-13), del(6)(p11), del(6)(q23), add(7)(p11), add(7)(q?11), der(7)t(7;16)(p11;q11), add(8)(p11), der(9)t(9;16)(p23;q11)x2, der(11)t(6;11)(?q23;p1?3), der(12)add(12)(p11)add(12)(q21), add(13)(p11), add(14)(p11), der(14)t(14;16)(p11;p11)der(14)t(14;20)(q32;q11), add(16)(q11), add(17)(p13), add(17)(q23), add(18)(p11)add(18)(q23), del(18)(q22), add(19)(q11), dup(19)(q11q13), der(20)t(14;20)(q32;q11), i(21q), der(22)add(22)(p11)add(22)(q13)x2, der(22)t(6;22)(q11;q12-13), inc - carries semi-cryptic t(14;20) effecting juxtaposition of IGH with MAFB |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |