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| 产品名称： | MOTN-1 |
|---|---|
| 商品货号： | TS259866 |
| <strong>Cell line </strong>： | MOTN-1 |
| DSMZ no. ： | TS259866 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 65-year-old woman with T cell large granular lymphocyte leukemia (T-CLL or T-LGL) in chronic phase in 2001. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14745, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single or clustered ovoid to round, sometimes irregularly shaped cells, growing in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS; the originator described that IL-2 supports growth of the cell line |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; max. density at ca. 2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximal cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, sm/cyCD3 -, CD4 +, CD5 -, CD6 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat moded near-diploid karyotype with 4% polyploidy - 40-47<2n>XX, (1;14)(p36;q12), der(2)t(X;2)(q37;q26), t(2;6)(q32;q24), t(11;17)(p15;q11), t(12;18)(q11;q21) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |