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| 产品名称： | HT |
|---|---|
| 商品货号： | TS259868 |
| <strong>Cell line </strong>： | HT |
| DSMZ no. ： | TS259868 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the ascitic effusion of a 70-year-old man with B cell non-Hodgkin lymphoma (B-NHL) in 1983 (diffuse large cell lymphoma); assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14375, 17355 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing as clumps in suspension; image |
| Medium ： | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3-4 days; maintain at 0.5-1 x 106 cells/ml; max. density at about 1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 3% polyploidy - 46(42-46)<2n>XY, +2, der(2;4)(p10;q10), der(2)(del)(2)(p11?q21), dup(10)(q11q22-23), dup(11)(?q23qter) - no IGH-BCL2 rearrangement detected |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |