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| 产品名称： | C2C12 |
|---|---|
| 商品货号： | TS259870 |
| <strong>Cell line </strong>： | C2C12 |
| DSMZ no. ： | TS259870 |
| Species ： | confirmed as mouse by PCR species and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | myoblasts |
| Origin ： | subclone of a mouse myoblast cell line established from mouse strain C3H; C2C12 cells are described in the literature to differentiate rapidly forming myotubes when incubated with horse serum; reportedly treatment with bone morphogenic protein 2 induces differentiation into the osteoblast lineage |
| Reference(s) ： | 14393, 14651 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing adherently in monolayers; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out 1-5 x 105 cells in 80 cm2; split semi-confluent culture 1:10 to 1:50 every 2-4 days using trypsin/EDTA (do not allow cultures to become confluent) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Cytogenetics ： | murine flat moded near-tetraploid karyotype |
| Viruses ： | PCR: SMRV - |