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| 产品名称： | MOLP-8 |
|---|---|
| 商品货号： | TS259871 |
| <strong>Cell line </strong>： | MOLP-8 |
| DSMZ no. ： | TS259871 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | multiple myeloma |
| Origin ： | established from the peripheral blood of a 52-year-old Japanese man with multiple myeloma (stage IIIA, type IgD lambda) in 2002 |
| Reference(s) ： | 14748 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells in suspension, some cells loosely adherent; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; max. density at about 1.5 x 106 cells/ml; cells should be grown in 24- or 12-well-plates; immediately after thawing viability may drop to 50%, but cells recover within a few days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype - 40-48<2n>X, -Y, -15, +1-2mar, t(3;8)(p22;q22), der(4)t(4;15)(q32;q21), inv(8)(q21q24), der(11)t(?4 ;11)(q31;q13), t(11;14)(q13;q32), t(16;18)(p13;q21), der(17)t(11;17)(q13;p13) - carries t(11;14) effecting IGH-CCND1 juxtaposition with additional MYC rearrangement present in inv(8) - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |