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| 产品名称： | NCI-H510A |
|---|---|
| 商品货号： | TS259873 |
| <strong>Cell line </strong>： | NCI-H510A |
| DSMZ no. ： | TS259873 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established in 1982 from an adrenal gland metastasis of a small cell lung cancer from a man in relapse following treatment; cells were described in the literature to be tumorigenic in nude mice and to express elevated levels of L-dopa decarboxylase, neuron-specific enolase, creatine kinase and bombesin-like immunoreactivity |
| Reference(s) ： | 14421 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + Hams F12 (at 1:1) + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 once a week using trypsin-like enzyme (e.g. Tryple Express) (standard trypsin/EDTA is not sufficiently effective); seed out 2-3 x 106 cells in 25 cm2; note that certain plastic flasks from some suppliers are inhibitory to growth, while others are conducive |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 1 week |
| Harvest ： | cell harvest of ca. 40 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |