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| 产品名称： | SU-DHL-8 |
|---|---|
| 商品货号： | TS259879 |
| <strong>Cell line </strong>： | SU-DHL-8 |
| DSMZ no. ： | TS259879 |
| Species ： | confirmed as human by species PCR and cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the pleural effusion of a 59-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse large cell lymphoma, noncleaved cell type and later as diffuse histiocytic lymphoma; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 15065, 14515, 14605, 17354, 14516 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells growing in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 2-3 days; maintain at ca. 1-2 x 106 cells/ml; maximal density at 3-4 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-72 hours |
| Harvest ： | cell harvest of ca. 3-4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD34 -, CD38 +, CD80 +, CD138 -, HLA-DR +, cyIgG +, cyIgM -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 3% polyploidy - 51(47-53)<2n>X, -Y, +7, +13, +20, +20, t(8;22)(q24;q11), del(20)(q13), der(22)t(1;22)(q11;p11)del(1)(q?32) - carries t(8;22) effecting IGL-MYC rearrangement - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |