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| 产品名称： | SU-DHL-6 |
|---|---|
| 商品货号： | TS259881 |
| <strong>Cell line </strong>： | SU-DHL-6 |
| DSMZ no. ： | TS259881 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peritoneal effusion of a 43-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse, mixed small and large cell type; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14515, 14605, 15065, 16554, 17354, 14516, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small single cells and in clusters, in suspension; image; image |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 3 days; seed out at ca. 0.8 x 106 cells/ml in a 24-well-plate; maintain at ca. 0.4-1.0 x 106 cells/ml, best in 12- or 24-well-plates; a certain amount of cell debris is always visible; a temporary reduction in cell viability may occur after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 hours |
| Harvest ： | cell harvest of ca. 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 2% polyploidy - 47(42-48)<2n>X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8;9)(q24;p13), der(9)t(8;19;9)(q24;q13;p13), dup(11)(q24q25), t(14;18)(q32;q21), der(22)t(?7;22)(?q32;p11) - sideline with dic(8;9)(q24;p13), ider(8)(q10)t(8;9(q24;p13) - matches published karyotype - carries t(14;18) effecting IGH-BCL2 fusion |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |