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| 产品名称： | CI-1 |
|---|---|
| 商品货号： | TS259885 |
| <strong>Cell line </strong>： | CI-1 |
| DSMZ no. ： | TS259885 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the ascites of a 38-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL, lymphoblastic, non-convoluted cell type) at diagnosis in 1981 |
| Reference(s) ： | 14951, 15013 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | clustered cells in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; max. density at about 1.5 x 106 cells/ml; cells should be grown in 24-well-plates during the first week after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 6% polyploidy - 46(44-49)<2n>XX, t(2;8)(p11;q24), t(14;22)(q32;q11) - matches published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |