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| 产品名称： | SU-DHL-16 |
|---|---|
| 商品货号： | TS259891 |
| <strong>Cell line </strong>： | SU-DHL-16 |
| DSMZ no. ： | TS259891 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the pleural effusion of a man with B-cell non-Hodgkin lymphoma (B-NHL) |
| Reference(s) ： | 15039, 15062 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing in clumps; image ; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:6 every 2-3 days; seed out at ca. 0.5-1.0 x 106 cells/ml in a 24-well-plate, after thawing viability may be less than 70% but the cells recover during the following 3-4 days; maintain at ca. 0.3-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD138 -, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 3% polyploidy - 47(44-49)<2n>XXY, +10, -13, +15, t(1;5)(q31;q21), add(2)(q21-22), der(2)t(2;2)(q3?7;q21-22)t(2;10)(p25;q24), der(10)t(2;10)(p25;q24)x2, del(12)(p12)dup(12)(q11q22), der(14)t(13;14)(q11;p11)t(14;18)(q32;q21), add(15)(q23)x2, i(17q), der(18)t(14;18)(q32;q21) - carries t(14;18) effecting BCL2-IGH rearrangement |
| Molec. Genetics ： | expression of fusion gene BCL2-IGH confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |