微信小李
微信小章
产品简介
购买须知
| 产品名称： | SU-DHL-10 |
|---|---|
| 商品货号： | TS259893 |
| <strong>Cell line </strong>： | SU-DHL-10 |
| DSMZ no. ： | TS259893 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the pleural effusion of a 25-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse histiocytic lymphoma; cell line carries EZH2 Y641F mutation (own results); assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14515, 14516, 17354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round lymphoid cells growing singly or in clumps in suspension; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.8 x 106 cells/ml in 12-well-plates; after thawing viability may be low, but cells will soon start to grow vigorously; split saturated culture 1:10 - 1:20 every 2-4 days; maintain at 0.03-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 15-30 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, HLA-DR +, cyIgG +, cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype - 47(43-48)<2n>XY, +7, der(8)t(X;8)(q25;p23)t(8;X)(q24;q26)t(X;14)(q28;q32), del(10)(q22q24), der(11)t(Y;11)(q11;q25), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - carries concurrent rearrangements of IGH with MYC and BCL2 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |