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| 产品名称： | SUP-HD1 |
|---|---|
| 商品货号： | TS259895 |
| <strong>Cell line </strong>： | SUP-HD1 |
| DSMZ no. ： | TS259895 |
| Species ： | originally confirmed as human by cytogenetics and species PCR, subsequently by DNA fingerprinting |
| Cell type ： | Hodgkin lymphoma |
| Origin ： | established from a 37-year-old man at second (refractory/terminal) relapse of Hodgkin lymphoma (nodular sclerosing -> lymphocyte depleted/stage IIISA -> stage IV) after both combined chemo- and radiotherapy in 1987. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14801, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round (some polygonal) cells growing singly or in small clumps in suspension, heterogeneity in cell size; image; image; image; image |
| Medium ： | 80% McCoys 5A + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 12- or 24-well-plates; maintain at ca. 0.3-0.6 x 106 cells/ml; split saturated culture 1:3 to 1:4 every 3-5 days; cells grow better in plates than flasks, though viabilities rarely exceed 90% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-80 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with MRA (Mycoplasma Removal Agent), then negative in microbiological culture, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD25 +, CD30 -, CD34 -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hyperdiploid karyotype with 4% polyploidy - 48(44-48)<2n>X, -Y, +5, +8, +9, -13, +19, +mar, der(1)t(1;1)(p34;q12.2), del(2)(p23), ins(2;8)t(p2?3;??), add(4)(p15), add(4)(q23), add(5)(p1?1), i(5p), der(5)t(5;15)(p10;p10), del(6)(p23), der(6)i(6)(q10)der(6)t(6;9)(q2?5;p2?1), der(8)add(8)(p11)add(8)(q2?4), der(8)t(8;12)(p22;?p12), del(9)(q3?2), der(9)t(9;12)(q31;?p12), i(9q), der(11)t(4;11)(?p15;p15), del(11)(q22.2), add(12)(p13), i(14q), der(15)t(2;15)(?p23;q26), i(18q), der(19)del(19)(p12)add(19(q13), del(21)(q22), der(22)t(13;22)(q31;q22.3) - sdl with del(1)(p32), i(14)(q10)del(14)(q11q13-21) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |