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| 产品名称： | TOM-1 |
|---|---|
| 商品货号： | TS259897 |
| <strong>Cell line </strong>： | TOM-1 |
| DSMZ no. ： | TS259897 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established in 1983 from the bone marrow of a 54-year-old woman with refractory Philadelphia chromosome-positive acute lymphoblastic leukemia (ALL); described in the literature to carry the ALL-variant (m-bcr) of the BCR-ABL1 fusion gene (e1-a2) |
| Reference(s) ： | 14822 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing in suspension singly or in clumps, some cells are loosely adherent; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells may be difficult to culture; seed out at ca. 1-2 x 106 cells/ml (it could be of advantage to start in 24-well-plates); also, after thawing viability may drop to about 40-50% but will recover after about one week, cells start to grow after ca. 5 days; split saturated culture 1:2 every 3 days; maintain at ca. 0.5-2.0 x 106 cells/ml; maximal density at about 2.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD4 -, CD7 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 +, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 7% polyploidy - 47(47-48)<2n>X, -X, +8, +16, del(7)(p14), der(9)del(9)(q13q34)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11) - carries t(9;22) effecting BCR-ABL1 rearrangement - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |