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| 产品名称： | HTC-C3 |
|---|---|
| 商品货号： | TS259898 |
| <strong>Cell line </strong>： | HTC-C3 |
| DSMZ no. ： | TS259898 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | thyroid carcinoma |
| Origin ： | established from cancer cells disseminated in the pleural fluid of a 44-year-old woman with undifferentiated giant cell carcinoma of the thyroid after chemotherapy, x-ray hyperthermia and OK432 injections in 1986; cells were described in the literature to contain parathyroid-like activity |
| Reference(s) ： | 14512 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayer ; image; image; image |
| Medium ： | 80% Dulbeccos MEM (4.5 g/L glucose) + 20% h.i. FBS |
| Subculture ： | seed out 0.2-0.5 x 106 cells in 80 cm2; split confluent culture 1:2 once a week using trypsin-like enzyme (e.g. Tryple Express) (standard trypsin/EDTA is not sufficiently effective); note that viability may decrease rapidly after reaching confluency |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | cell harvest of ca. 12 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-triploid karyotype with 23% polyploidy - 68(68-73)<3n>XXXXX, add(X)(p11), add(X)(p11)add(X)(q11), add(X)(q11), del(X)(p11), der(6)t(X;6)(q23;q25), inc. |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |