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| 产品名称： | NU-DHL-1 |
|---|---|
| 商品货号： | TS259900 |
| <strong>Cell line </strong>： | NU-DHL-1 |
| DSMZ no. ： | TS259900 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the left inguinal lymph node of a 73-year-old Caucasian man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma, non-cleaved cell type) in 1982; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14517, 15065, 17354, 17355, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to polymorph cells growing in suspension; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out and maintain at ca. 0.5-1.0 x 106 cells/ml; it is recommended to start culture in a 24-well-plate and with 20% FBS; split saturated culture 1:2 to 1:4 every 2-3 days; viability of culture may drop to about 50% after thawing and may need more than one week to recover |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | maximum density at about 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, fm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 51(46-53)<2n>XY, +5, +8, +9, +12, +12, t(3;8)(p25;q24), i(5p), dup(7)(q21.3q31.1), der(8)t(3;8)(p25;q24), i(9p), del(12)(q11), i(12p), der(14)t(14;18)(q32;q21)x1-3 - sideline with dup(2)(p2?1p2?4) - carries t(3;8) and t(14;18) effecting respective rearrangements of MYC and IGH-BCL2 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |