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| 产品名称： | 8709 |
|---|---|
| 商品货号： | TS259908 |
| <strong>Cell line </strong>： | 8709 |
| DSMZ no. ： | TS259908 |
| Species ： | originally confirmed as mouse by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the splenocytes of a radiation-induced acute myeloid leukemia (AML) of the C3H inbred mouse strain in 1997 |
| Reference(s) ： | 14534, 15205 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells growing singly or in small clusters in suspension, partly slightly adherent |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 10 ng/ml muIL-3 or 10-20% WEHI-3B supernatant |
| Subculture ： | split culture 1:2 twice a week; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.0 x 106 cells/ml; after thawing it is advisable to culture the cells first in 24-well plates and to add 10 ng/ml mIL-3 or 10-20% WEHI-3B supernatant daily; viability may be low after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | maximum density at about 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Viruses ： | PCR: SMRV - |