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| 产品名称： | 7926 |
|---|---|
| 商品货号： | TS259914 |
| <strong>Cell line </strong>： | 7926 |
| DSMZ no. ： | TS259914 |
| Species ： | Originally confirmed as mouse by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | acute myeloid leukemia |
| Origin ： | murine radiation-induced acute myeloid leukemia (AML) cell line derived from splenocytes of the C3H inbred mouse strain in 1997; cell line was described in the literature to be radio-sensitive |
| Reference(s) ： | 14534, 15204, 15205 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing in clumps or singly in suspesion |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + (10 ng/ml mIL-3) |
| Subculture ： | split saturated culture 1:2 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; cells are very difficult to culture, mIL-3 supports growth of the cells, cells grow best in 12- or 24-well-plates; after thawing, for the first 7-10 days there is virtually no cell growth or cells grow very slowly, viability may be initially very low, not exceeding 80-85% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-80 hours |
| Harvest ： | maximum density at about 1-1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Viruses ： | PCR: SMRV - |