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| 产品名称： | RI-1 |
|---|---|
| 商品货号： | TS259915 |
| <strong>Cell line </strong>： | RI-1 |
| DSMZ no. ： | TS259915 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood of a 57-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL, lymphocytic, small cell type progressing to large non-convoluted cell type) in the refractory terminal stage in 1977; assigned to ABC-like lymphoma subtype (activated B-cell); cell line also known as Riva. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15013, 17354, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large round cells growing in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 1-2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-72 hours |
| Harvest ： | maximum density at about 3-4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 4% polyploidy - 48-51<2n>XX, +7, +18, +19, +mar, t(1;22;16)(p35;p12;q12.2), del(3)(q11-12), trp(3)(q11-12q28-29), t(4;8)(q22;q24), del(6)(q13), der(15)t(13;15)(q11;p1?1), der(18)amp(18)(q21)dup(18)(q21q23), der(19)t(6;19)(p21;p13)add(19(q13.3) - carries t(4;8) and der(18) effecting MYC rearrangement and amplification of BCL2, respectively - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |