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| 产品名称： | REC-1 |
|---|---|
| 商品货号： | TS259917 |
| <strong>Cell line </strong>： | REC-1 |
| DSMZ no. ： | TS259917 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the lymph node or peripheral blood from a 61-year-old man with terminal refractory B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell progressing to transformed mantle cell, blastoid variant). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14885, 14892, 15229, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or in clumps in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.6- 1.0 x 106 cells/ml; maintain at ca. 0.4-1.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; recommended to start culture after thawing with 20% FBS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at about 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR -, sm/cyIgG -, sm/cy IgM +, sm/cykappa +, sm/cy lambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded near-diploid karyotype with 25% polyploidy - 41-47<2n>XY/XXY, -2, -8, +11, +12, -13, -22, add(5)(p15), i(7q), add(9)(p22), der(9)t(9;17)(q3?1;q11-12), add(10)(q24-q25), add(11)(q11), t(11;14)(q13;q32), add(12)(q22), der(12)t(8;12)(q11;p12), der(12)t(12;16)(p11;p11), der(16)t(16;17)(p11;?q25)t(2;16)(p1?2;q2?1), der(16)t(16;22)(p13;q11), add(17)(q25)x2 - carries t(11;14) effecting rearrangement of IGH with CCND1/BCL1 - near tetraploid sideline with additional rearrangements present - matches published description |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |