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| 产品名称： | AP-1060 |
|---|---|
| 商品货号： | TS259923 |
| <strong>Cell line </strong>： | AP-1060 |
| DSMZ no. ： | TS259923 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the bone marrow of a 45-year-old man with acute promyelocytic leukemia (APL = AML FAB M3) in fourth relapse in 1998; cells were immortalized using ethyl-nitrosourea; cells are described in the literature to be constitutively dependent on GM-CSF and IL-3 or G-CSF; cells carry and express the t(15;17)(q22;q11.2) PML-RARA fusion gene and the t(12;15)(p13;q25) ETV6-NTRK3 fusion gene |
| Reference(s) ： | 14994, 18148 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension; image; image; image |
| Medium ： | 70% Iscoves MDM + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSM ACC 35) |
| Subculture ： | cells are difficult to culture! Seed out at ca. 1 x 106 cells/ml in a 24-well-plate; split saturated culture 1:2 or less once or twice a week; maintain at ca. 1.0-1.5 x 106 cells/ml; use always 24- or 12-well-plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | maximum density at about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 3% polyploidy - 46(42-47)<2n>XY, t(3;14)(p21;q11.2), add(5)(q33), del(6)(q25), add(12)(p13), t(15;17)(q22;q21); carries t(15;17) with rearrangement of PML and RARA; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene PML-RARA (L-form) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |