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| 产品名称： | MOLM-20 |
|---|---|
| 商品货号： | TS259924 |
| <strong>Cell line </strong>： | MOLM-20 |
| DSMZ no. ： | TS259924 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | chronic neutrophilic leukemia |
| Origin ： | established from the peripheral blood of a 63-year-old woman with chronic neutrophilic leukemia (CNL) in transformation/blast crisis in 2003; MOLM-20 was established independently in another laboratory and is a sister cell line of CNLBC1 (see references). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14684, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture; seed out at ca. 2 x 106 cells/ml in a 24-well-plate; maintain at ca. 1-2 x 106 cells/ml in a 24-well-plate; split saturated culture 1:2 once or twice per week; post thawing cells may need 5-7 days to recover and to reach viabilities above 80% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | maximum density at about 4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile which matches the profile of the sister cell line CNLBC1 |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy; 49(47-50)<2n>XXX, +X, +8, +21, t(4;11)(q21.1;q23); carries MLL rearrangement with 4q21.1 breakpoint outside (centromeric) of AFF1 (AF4); matches karyotype of sister cell line CLNBC1 |
| Molec. Genetics ： | expression of fusion gene KMT2A-SEPT11 (MLL-SEPT11; MLL-FLJ10849) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |