微信小李
微信小章
产品简介
购买须知
| 产品名称： | BEN-MEN-1 |
|---|---|
| 商品货号： | TS259929 |
| <strong>Cell line </strong>： | BEN-MEN-1 |
| DSMZ no. ： | TS259929 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | benign meningioma |
| Origin ： | established in 2003 from the meningothelial meningioma (grade I, WHO, #1238-03) attached to the parietal falx of a 68-year-old woman after surgical tumor resection; cells were immortalized by retroviral transduction with human telomerase reverse transcriptase (hTERT) |
| Reference(s) ： | 14864 |
| Biosafety level ： | 1, GMO-S1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelioid cells growing as monolayers; image; image |
| Medium ： | 80% Dulbecco´s MEM + 20% h.i. FBS |
| Subculture ： | optimal split ratio 1:2 to 1:5 every 2-6 days; seed out at ca. 0.7-2.0 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 12% polyploidy; 44(43-46)<2n>XX, -22; frequent random chromosome loss; monosomy 22 is recurrent in grade I benign meningiomas; matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |