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| 产品名称： | JL-1 |
|---|---|
| 商品货号： | TS259930 |
| <strong>Cell line </strong>： | JL-1 |
| DSMZ no. ： | TS259930 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | epithelioid mesothelioma |
| Origin ： | established from the untreated malignant epithelioid mesothelioma of the pleura of a 54-year-old man with asbestos cancer in 2003 |
| Reference(s) ： | 14850 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing in monolayers |
| Medium ： | 80% Dulbeccos MEM or NCTC-109 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:5 once or twice a week using trypsin/EDTA; seed out at ca. 2 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109) |
| Doubling time ： | ca. 70-80 hours |
| Harvest ： | cell harvest of ca. 5-7 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded karyotype with 12 % polyploidy; 42-47<2n>XY, +5, +7, -10, -13, +20, der(1)t(1;15)(q11;q11), inv(3)(p1?q2?), der(5)t(5;16)(q11;?), add(10)(q25), der(11)t(10;11)(q21;q23), der(14)t(1;14)(q11;q31); sdl with add(9)(p11), del(16)(q11); karyotype displays significant intercellular differences; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |