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| 产品名称： | BLUE-1 |
|---|---|
| 商品货号： | TS259936 |
| <strong>Cell line </strong>： | BLUE-1 |
| DSMZ no. ： | TS259936 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the bone marrow of a 29-year-old Caucasian man with Burkitt lymphoma in second relapse following combined chemotherapy and radiotherapy in 2004; cells are described in the literature to carry t(6;20)(q15;q11.2) leading to BACH2-BCL2L1 fusion gene |
| Reference(s) ： | 14413, 16135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clumps in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 0.5 - 2.0 x 106 cells/ml; split 1:2 to 1:5 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at about 3.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, cykappa -, cylambda + |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype; 53(49-53)<2n>XY, +6, +13, +16, +20, +20, +20, +21, t(6;20)(q15;q11.2), der(6)t(6;20)(q15;q11.2), t(8;14)(q24;q32), der(20)del(20)(p12.2p13.2)t(6;20)(q15;q11.2)t(6;11)(q16;p13)x2; carries t(8;14) effecting IGH-MYC juxtaposition; matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |