微信小李
微信小章
产品简介
购买须知
| 产品名称： | HNT-34 |
|---|---|
| 商品货号： | TS259938 |
| <strong>Cell line </strong>： | HNT-34 |
| DSMZ no. ： | TS259938 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established in 1994 from the peripheral blood of a 47-year-old woman with acute myeloid leukemia (AML FAB M4) secondary to previous myelodysplastic syndromes (MDS), specifically chronic myelomonocytic leukemia (CMML); described in the literature to carry the t(3;3)(q21;q26) leading to MECOM (EVI1) overexpression and the Philadelphia chromosome t(9;22) expressing both P190 and P210 BCR-ABL1 chimeric transcripts |
| Reference(s) ： | 14593 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and in clusters in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.8 x 106 cells/ml initially in a 24-well-plate; viability may drop below 50% during the first few days and a significant amount of cell debris is always visible; in logarithmic growth phase split culture 1:2 to 1:3 every 2-3 days and maintain at ca. 0.5-2.0 x 106 cells/ml; maximal density at about 3 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 2-3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD15 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype with 7% polyploidy; 46(44-48)<2n>XX, t(3;3)(q21;q26), t(9;22)(q34;q11), del(17)( ?p12.2p13.1), del(20)(q13.1q13.2); t(3;3) and t(9;22) effect rearrangement of MECOM (EVI1) and BCR-ABL1, respectively; matches published karyotype. |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |