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| 产品名称： | RS-5 |
|---|---|
| 商品货号： | TS259939 |
| <strong>Cell line </strong>： | RS-5 |
| DSMZ no. ： | TS259939 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | sarcomatoid mesothelioma |
| Origin ： | established in 2003 from the untreated malignant mesothelioma of the pleura of a 78-year-old man with asbestos cancer |
| Reference(s) ： | 14850 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, sarcomatoid-like cells growing in monolayers; image; image; image |
| Medium ： | 90% NCTC-109 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:4 once a week using trypsin/EDTA; cells grow slowly for about one week after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3 days |
| Harvest ： | maximum density of ca. 3 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype with 5% polyploidy; 46(39-48)<2n>XY; although no consistent abnormality was detected, most metaphases showed random chromosome loss |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |