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| 产品名称： | L-591 |
|---|---|
| 商品货号： | TS259942 |
| <strong>Cell line </strong>： | L-591 |
| DSMZ no. ： | TS259942 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B lymphoblastoid cells (from patient with Hodgkin lymphoma) |
| Origin ： | established from the pleural effusion of a 31-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB) in 1982 |
| Reference(s) ： | 14463, 15213 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in loosely-attached clusters |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split culture 1:2 twice a week; seed out at ca. 0.5 x 106 cells/ml in 24-well-plates; optimal growth is achieved using 24-well-plates, but culture can be adapted to grow in flasks with lower viabilities; maintain at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 72 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 -, CD8 -, CD10 -, CD19 +, CD20 -, CD21 +, CD25 +, CD30 +, CD34 -, CD80 +, CD138 -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 6% polyploidy; 46(45-48)<2n>XX, add(7)(q32), der(14)t(9;14)(p13;q32); der(14) effects rearrangement of PAX5 which (untypically for HL) is upregulated |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |