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| 产品名称： | KYO-1 |
|---|---|
| 商品货号： | TS259943 |
| <strong>Cell line </strong>： | KYO-1 |
| DSMZ no. ： | TS259943 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the peripheral blood of a 22-year-old Japanese man with chronic myeloid leukemia (CML) in myeloid blast crisis in 1981 |
| Reference(s) ： | 14814 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clumps in suspension; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split culture 1:2 to 1:4 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD14 -, CD19 -, CD33 +, CD34 -, CD41 -, CD42b -, CD71 +, CD235a +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype; 83-90<4n>XX, -Y, -Y, +5, +8, -9, -9, +11, +11, +15, -16, -17, -17, -18, -18, +22, +22, +2mar; t(3;12)(p12.2;p11-12) x2, der(5)t(5;6)(p15;?q22)t(6;12)(q2?;?)ins(6;12)(q2?;?), del(6)(q22) x2, der(8)t(8;9)(q25;p11-12) x2, der(9)add(9)(p11-12)t(9;22)(q34;q11) x2, add(11)(q11) x2, der(12)t(6;12)(?;?) x2, der(15)t(15;22)(p11;q11) x2, der(17)t(17;17)(p12-13;q23) x2, der(22)t(9;22)(q34;q11) x4; carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |