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| 产品名称： | RAMOS |
|---|---|
| 商品货号： | TS259946 |
| <strong>Cell line </strong>： | RAMOS |
| DSMZ no. ： | TS259946 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described in the literature to be EBV-negative but EBV infectible, to carry the (8;14) translocation and TP53 mutations. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14669, 14527, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clumps in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.8 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; medium must be changed frequently to prevent acidification and the specified cell density should be complied |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype; 45(44-46)<2n>X, -Y, del(2)(p16p23), t(8;14)(q24;q32.2), add(14)(p11), der(16)t(7;16)(q11.2;p13.2), der(17)t(13;17)(q33;p11.3)t(13;3)(q13;q?25), del(20)(q12); carries t(8;14) effecting IGH-MYC rearrangement |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |