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| 产品名称： | R3327-MATLyLu |
|---|---|
| 商品货号： | TS259951 |
| <strong>Cell line </strong>： | R3327-MATLyLu |
| DSMZ no. ： | TS259951 |
| Species ： | confirmed as rat by species PCR |
| Cell type ： | prostate tumor |
| Origin ： | established from tumor material of a previously subcutaneously passaged rapidly growing, hormone independent, anaplastic and metastatic variant of the rat Dunning R 3327 AT prostate tumor in Copenhagen rats; cells were described in the literature to be androgen insensitive and to have a high metastatic potential with dissemination to lymph nodes and lungs in rats |
| Reference(s) ： | 14551, 15142 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing adherently in monolayers |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | split culture 1:10 to 1:20 every two days using trypsin/EDTA; seed out at ca. 2 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 15 hours |
| Harvest ： | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Viruses ： | PCR: SMRV - |