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| 产品名称： | SET-2 |
|---|---|
| 商品货号： | TS259954 |
| <strong>Cell line </strong>： | SET-2 |
| DSMZ no. ： | TS259954 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | essential thrombocythemia |
| Origin ： | established from the peripheral blood of a 71-year-old woman with essential thrombocythemia at megakaryoblastic leukemic transformation in 1995; cells were described in the literature to carry the JAK2 V617F mutation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14871, 15030, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single cells in suspension, some cells grow loosely adherent, 1-3% giant cells; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | start and cultivate in 24- or 12-well plates for optimal growth; seed out at ca. 0,8 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split culture 1:2 every 3-4 days; possibly no cell growth during first week after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD38 +, CD71 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype with 8% polyploidy; 46-47<2n>XX, +13, -14, +21, +mar, add(4)(p15), der(5)t(5;19)(q11;q13), der(7)t(7;14)(q31;q11), del(9)(p11p24)ins(9;?21)(p11;p11p12), add(9)(q11)t(9;22)(p24;q12), der(13)del(13)(q11-q13)t(13;21)(q34;q11-12), der(13)t(13;21)(q34;q11-12), ins(16;9)(q22;?p23p24), der(19)t(9;19)(p24;q13), ider(?21;9)(p10p13;p24), der(21)r(21;9)(p1?1q1?1;p24p2?3); carries multiple rearrangements effecting 9p24 amplification; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |