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| 产品名称： | RL |
|---|---|
| 商品货号： | TS259959 |
| <strong>Cell line </strong>： | RL |
| DSMZ no. ： | TS259959 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1983 from the ascites of a 52-year-old man with B-NHL (diffuse, undifferentiated, small non-cleaved large cell) |
| Reference(s) ： | 14375 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoid cells growing singly or in clumps in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 0.7-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 5-8 days |
| Harvest ： | maximum density at ca. 1.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 16% polyploidy; 49(45-51)<2n>XY, +7, +17, +21, der(3)inv(3)(p24q24-q25)t(3;13)(q24-q25;q32), add(5)(p11), der(13)t(3;13)(q24-q25;q32), t(14;18)(q32;q21), der(17)dup(17)(p12p13)t(17;20)(q11.2;q11.2), r(17), add(19)(q12), der(19)t(19;20)(p13;p11), der(20)t(17;20)(q11.2;q11.2)x1-2; carries t(14;18) effecting rearrangement of BCL2 with IGH (IGH); resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |