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| 产品名称： | WSU-FSCCL |
|---|---|
| 商品货号： | TS259960 |
| <strong>Cell line </strong>： | WSU-FSCCL |
| DSMZ no. ： | TS259960 |
| Species ： | originally confirmed as human by species PCR, subsequently by DNA fingerprinting |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood of a 37-year-old white man with low-grade follicular small cleaved cell lymphoma (B-NHL, stage IVB) after chemotherapy and transformation to small cell non-cleaved non-Hodgkin´s lymhoma in 1989; described in the literature to carry t(14;18) and t(8;11) with BCL2 and MYC rearrangements, respectively |
| Reference(s) ： | 14969, 14774, 14775 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at 0.25-4.0 x 106 cells/ml; optimal split ratio of 1:3 to 1:5 about 2-3 times a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-30 hours |
| Harvest ： | maximum density at ca. 4.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy; 47(46-50)<2n>XY, -13, +2mar, add(1)(p34), del(6)(q25), t(8;11)(q24.3;q24), i(13q), t(14;18)(q32;q21); t(14;18) effects IGH-BCL2 rearrangement |
| Molec. Genetics ： | expression of fusion gene IGH-BCL2 (MBR) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |