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| 产品名称： | MOLM-6 |
|---|---|
| 商品货号： | TS259961 |
| <strong>Cell line </strong>： | MOLM-6 |
| DSMZ no. ： | TS259961 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the peripheral blood of a 44-year-old man with Philadelphia chromosome-positive chronic myeloid leukemia (CML) in blast crisis in 1992 |
| Reference(s) ： | 15023 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; some cells may be adherent; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 1-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-72 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat moded near triploid karyotype; 62-77<3n>XY/XXY, +6, +6, -14, -15, -16, -17, -18, +22, +22, +2mar, t(9;22)(q34;q11), i(11q), der(22)t(9;22)(q34;q11)x3; add(7)(p11) present in sdl; cells typically carry 4 copies of der(22)t(9;22) / Ph effecting BCR-ABL1 rearrangement |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |