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| 产品名称： | HAL-01 |
|---|---|
| 商品货号： | TS259965 |
| <strong>Cell line </strong>： | HAL-01 |
| DSMZ no. ： | TS259965 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 17-year-old woman with acute lymphoblastic leukemia (ALL-L2) in 1990; contains the t(17;19)(q22;p13) TCF3-HLF (E2A-HLF) fusion gene |
| Reference(s) ： | 14744, 14820, 18196 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension, some cells are slightly adherent; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 1-2 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 twice a week; adherent cells can be detached by tapping the flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 4% polyploidy; 46(43-48)<2n>XX, t(1;7)(p34;q21), t(17;19)(q22;p13); t(17;19) effects rearrangement of TCF3 (E2A) and HLF; matches published karyotype |
| Molec. Genetics ： | expression of fusion gene TCF3-HLF (E2A-HLF) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |