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| 产品名称： | FKH-1 |
|---|---|
| 商品货号： | TS259968 |
| <strong>Cell line </strong>： | FKH-1 |
| DSMZ no. ： | TS259968 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | acute myelocytic leukemia |
| Origin ： | established in 1993 from the peripheral blood of a 61-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) with trilineage myelodysplasia at refractory leukemic transformation into acute myeloid leukemia (AML M4); carries t(6;9)(p23;q34) leading to DEK-NUP214 (DEK-CAN) fusion gene; cells do not respond to G-CSF, as originally described |
| Reference(s) ： | 14594 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single, round cells in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml in 24- or 12-well plates; optimal split ratio of 1:2 or less every 3-4 days; cultures may contain a high amount of cell debris; the accumulation of cell debris can be prevented by using new culture vessels every second week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-100 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD11b +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy; 45(43-46)<2n>XY, -7, t(6;9)(p23;q34); carries t(6;9) effecting rearrangement of DEK with NUP214 (CAN); closely resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |