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| 产品名称： | SCC-4 |
|---|---|
| 商品货号： | TS259972 |
| <strong>Cell line </strong>： | SCC-4 |
| DSMZ no. ： | TS259972 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established from the squamous cell carcinoma of the tongue from a 55-year-old man after radio- and chemotherapy in 1980 |
| Reference(s) ： | 14889 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 40 ng/ml hydrocortisone |
| Subculture ： | seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum density at ca. 4 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded heteroploid karyotype with pentaploid and decaploid peaks; 100-117/202-266<5N/10N>XXYY/XXXXYYYY, add(Y)(q12), add(2)(q23), add(3)(q28), del(3)(p11), add(4)(p15), ins(4;?)(q32;??), incomplete |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |