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| 产品名称： | GF-D8 |
|---|---|
| 商品货号： | TS259974 |
| <strong>Cell line </strong>： | GF-D8 |
| DSMZ no. ： | TS259974 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | acute myelocytic leukemia |
| Origin ： | established from the peripheral blood of an 82-year-old man with acute myelocytic leukemia (AML M1) at diagnosis in 1989; described in the literature to be growth-dependent on granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3) |
| Reference(s) ： | 14879 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorph cells growing in suspension; image; image |
| Medium ： | 70% RPMI 1640 + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 50 ng/ml of human recombinant GM-CSF) |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well plates; maintain at 0.3-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days; cells grow slowly for about one week after thawing. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-72 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, HLA-DR -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype; 49(45-51)<2n>XY, +8, +13, +21, +mar, der(5)t(5;15)(q11;q25), der(7)t(7;15)(q31;q24), ?inv(7)(q31.3q35)x2, der(8)ins(8;11)(q24;q23q24)dup(8)(q23q24)dup(11)(q22q23), der(11)t(8;11)(q24;q23)dup(8)(q23q24)dup(11)(q22q23), der(11;17)(q10;q10), add(12)(p13), der(14)t(5;14)(q32;q24); resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |