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| 产品名称： | OCI-M2 |
|---|---|
| 商品货号： | TS259984 |
| <strong>Cell line </strong>： | OCI-M2 |
| DSMZ no. ： | TS259984 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | acute myelocytic leukemia |
| Origin ： | established in 1984 from the leukemic cells of a 56-year-old patient with erythroleukemia (AML-M6), representing the end stage of a previously identified myelodysplastic syndrome; described in the literature to differentiate along the erythroid or megakaryocytic/monocytic lineage after stimulation with erythroid inducers or phorbol esters, respectively; described in the literature to constitutively and inducibly secrete platelet-derived growth factor (PDGF)-like proteins; contamination with Mycobacterium was eliminated with kanamycin. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14833, 14834, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clusters in suspension, occasionally a few cells become adherent; image; image; image; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at 1-1.5 x 106 cells/ml; optimal split ratio 1:3 to 1:5 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD71 +, CD235a +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy; 51(46-51)<2n>XX, +6, +8, +20, -21, +3mar, der(X)t(X;8)(q23;q23), add(9)(p23)del(9)(p12p21), t(10;12)(p12;p12), del(17)(q11q21.1); no reference karyotype available |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |