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| 产品名称： | U-HO1 |
|---|---|
| 商品货号： | TS259991 |
| <strong>Cell line </strong>： | U-HO1 |
| DSMZ no. ： | TS259991 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | Hodgkin lymphoma |
| Origin ： | established in 2005 from the pleural effusion of a 23-year-old man with terminal refractory classical Hodgkin lymphoma (nodular sclerosing type) after radiotherapy, salvage chemotherapy and autologous stem cell transplantation; described in the literature to carry an amplification of REL-BCL11A region with overexpression of miR-216/217 |
| Reference(s) ： | 14728 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing singly and in clumps in suspension; occasionally large cells present; image; image; image |
| Medium ： | 80% Iscoves MDM + RPMI 1640 (at 4:1) + 20% h.i. FBS, 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-1.5 x 106 cells/well in 24-well-plate; optimal split ratio 1:2 every 3-5 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 +, CD34 -, HLA-DR +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 9% polyploidy; 51(46-52)<2n>XY, +5, +6, +8, +12, +17, +20, -22, +mar, del(1)(p13p31), der(2)qdp(2)(p1?5p1?6)t(2;10)(p24;q25.2), add(4)(q35), t(4;6)(p15.2;p21), del(5)(q11q34), der(5)t(5;15)(p11;q13), t(5;22)(q35;q13), del(6)(q13), der(10)t(2;10)(p24;q25.2)x1-2, t(12;18)(p10;q10), del(15)(q11q14), add(15)(q14), del(20)(q13)x1-2, dup(21)(q22.1q22.2), der(21)t(15;21)(q15;p11); genomic amplification of BCL11A-REL region on der(2); resembles published karyotype. |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |