微信小李
微信小章
产品简介
购买须知
| 产品名称： | ULA |
|---|---|
| 商品货号： | TS259993 |
| <strong>Cell line </strong>： | ULA |
| DSMZ no. ： | TS259993 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 2002 from the mononuclear ascites cells of a 57-year-old man with terminal diffuse large B cell lymphoma (stage IV) after failing to respond to several rounds of chemotherapy (CHOP, ICE) |
| Reference(s) ： | 14385 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing as single cells or in small clumps in suspension or slightly adherent; when cultured on extracellular matrix (Matrigel) cells grow adherently; image; image |
| Medium ： | 80% Iscove´s MDM + Optimem (at 1:1) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:2 to 1:5 every 2-3 days; to increase viability flasks may be coated with Matrigel (dilute 1:3 - 1:5 in Optimem, incubate for 2 hours at room temperature, then carefully remove excess medium and wash two times gently with PBS) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | maximum density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy 47(42-48)<2n>XY, +7, +8, -15, der(3)del(3)(p21p22)t(3;8)(q27;q24.1), t(3;8)(q27;q24.3), t(4;20)(q21;p12), t(7;12)(q22;p13), der(8)t(3;8)(q27;q24.3), der(8)t(3;8)(q27;q23)t(8;7)(q24.3;q11), inv(9)(p21q34), del(10)(q22), t(14;18)(q32;q21), del(19)(q13.3); sdl without t(4;20); carries t(14;18) effecting IGH-BCL2 rearrangement; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |