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| 产品名称： | BL-2 |
|---|---|
| 商品货号： | TS259995 |
| <strong>Cell line </strong>： | BL-2 |
| DSMZ no. ： | TS259995 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established in 1979 from the bone marrow of a 7-year-old Caucasian boy with non endemic Burkitt lymphoma (stage III) after chemo- and radiotherapy; cells are described in the literature to express no Epstein-Barr virus (EBV) antigen and that somatic immunoglobulin gene hypermutation can be induced |
| Reference(s) ： | 14387, 14454, 14502, 14526 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, round to polymorphic cells, growing in suspension; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; optimal split ratio 1:3 to 1:5 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-diploid karyotype with 3% polyploidy; 44-47<2n>XY, der(1)t(1;7)(q32;q11.2), der(6)t(1;6)(q21;q25), t(8;22)(q24;q11.2), del(11)(q24.2); carries t(8;22) effecting juxtaposition of MYC with IGL; matches published karyotype |
| Molec. Genetics ： | IGL-MYC translocation was verified by genomic PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |