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| 产品名称： | DOGKIT |
|---|---|
| 商品货号： | TS260005 |
| <strong>Cell line </strong>： | DOGKIT |
| DSMZ no. ： | TS260005 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the peripheral blood of a 56-year-old Caucasian man with Burkitt lymphoma after second relapse; described in the literature to carry t(8;22) and t(14;18) associated with MYC and BCL2 rearrangements, respectively |
| Reference(s) ： | 14659, 14404 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-1.0 x 106 cells/ml; optimal split ratio 1:2 to 1:8 every 2-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximum density at ca. 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 3.5% polyploidy; 46-49<2n>XY, +20, dup(1)(q21q42), der(2)t(2;14)(q37;q24)t(14;18)(q32;q21), t(8;22)(q24;q11.2), der(14)t(X;14)(p21;p13)t(14;18)(q32;q21), dup(17)(q11q22), der(18)t(14;18)(q32;q21); supernumerary acf/dm present at 14%; sdl with add(16)(p13); resembles published karyotype; carries t(8;22) and t(14;18) rearrangements effecting IGL-MYC and IGH-BCL2 rearrangements, respectively |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |