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| 产品名称： | U-2932 |
|---|---|
| 商品货号： | TS260008 |
| <strong>Cell line </strong>： | U-2932 |
| DSMZ no. ： | TS260008 |
| Species ： | confirmed as human by species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14356, 17355, 18133, 18134, 18135, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly and in clusters in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:3 twice a week, maintain at 1-1.5 x 106 cells/ml; could be of advantage to start culture in a 12- or 24-well plate |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human polyclonal hypodiploid karyotype with 20% polyploidy; 45(43-46)<2n>, XX, add(X)(q22), add(1)(q24), der(3)ins(3;18)(q27;q2?q2?)hsr(18)(q21), add(5)(q32), der(6)t(6;18)(p24;?q23)del(6)(q13), der(10)t(10;14)(q24;q23), der(11)t(1;11)(q25;q2?), der(14)t(3;14)(q27;p11), der(18)t(1;18)(q21;q21), der(18)t(3;18)(q2?;q27), add(19)(p13), del(19)(q13); sdl with del(2)(q11), t(4;15)(q22;q14), add(7)(q21), del(13)(?q21), der(13)add(13)(p11)add(13)(q32); extensive genomic amplification of BCL2 region; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |